5.5-Mechanisms of Other Antimicrobial Drugs

10.3 Mechanisms of Other Antimicrobial Drugs

Learning Objective

  • Explain the differences between modes of action of drugs that target fungi, protozoa, helminths, and viruses

Because fungi, protozoa, and helminths are eukaryotic, their cells are very similar to human cells, making it more difficult to develop drugs with selective toxicity. Additionally, viruses replicate within human host cells, making it difficult to develop drugs that are selectively toxic to viruses or virus-infected cells. Despite these challenges, there are antimicrobial drugs that target fungi, protozoa, helminths, and viruses, and some even target more than one type of microbe. Table 10.7Table 10.8Table 10.9, and Table 10.10 provide examples for antimicrobial drugs in these various classes.

Antifungal Drugs

The most common mode of action for antifungal drugs is the disruption of the cell membrane. Antifungals take advantage of small differences between fungi and humans in the biochemical pathways that synthesize sterols. The sterols are important in maintaining proper membrane fluidity and, hence, proper function of the cell membrane. For most fungi, the predominant membrane sterol is ergosterol. Because human cell membranes use cholesterol, instead of ergosterol, antifungal drugs that target ergosterol synthesis are selectively toxic (Figure 10.7).

The predominant sterol found in human cells is cholesterol, whereas the predominant sterol found in fungi is ergosterol, making ergosterol a good target for antifungal drug development.
Figure 10.7 The predominant sterol found in human cells is cholesterol, whereas the predominant sterol found in fungi is ergosterol, making ergosterol a good target for antifungal drug development.

Beyond targeting ergosterol in fungal cell membranes, there are a few antifungal drugs that target specific substances found in fungal cell walls, interfere with fungal cell division, or act as antimetbolites against fungal processes.

Table 10.7 shows the various therapeutic classes of antifungal drugs, categorized by mode of action, with examples of each.

Common Antifungal Drugs

Mechanism of Action

Drug Class

Specific Drugs

Clinical Uses

Inhibit ergosterol synthesis

Imidazoles

Miconazole, ketoconazole, clotrimazole

Fungal skin infections and vaginal yeast infections

Triazoles

Fluconazole

Systemic yeast infections, oral thrush, and cryptococcal meningitis

Allylamines

Terbinafine

Dermatophytic skin infections (athlete’s foot, ring worm, jock itch), and infections of fingernails and toenails

Bind ergosterol in the cell membrane and create pores that disrupt the membrane

Polyenes

Nystatin

Used topically for yeast infections of skin, mouth, and vagina; also used for fungal infections of the intestine

Amphotericin B

Variety systemic fungal infections

Inhibit cell wall synthesis

Echinocandins

Caspofungin

Aspergillosis and systemic yeast infections

Not applicable

Nikkomycin Z

Coccidioidomycosis (Valley fever) and yeast infections

Inhibit microtubules and cell division

Not applicable

Griseofulvin

Dermatophytic skin infections

Table 10.7

image
  • How is disruption of ergosterol biosynthesis an effective mode of action for antifungals?

Antiprotozoan Drugs

There are a few mechanisms by which antiprotozoan drugs target infectious protozoans (Table 10.8). Some are antimetabolites, others interfere with nucleic acid synthesis, and one class interferes with heme detoxification, which is necessary for the parasite’s effective breakdown of hemoglobin into amino acids inside red blood cells.

Common Antiprotozoan Drugs

Mechanism of Action

Drug Class

Specific Drugs

Clinical Uses

Inhibit electron transport in mitochondria

Naphthoquinone

Atovaquone

Malaria, babesiosis, and toxoplasmosis

Inhibit folic acid synthesis

Not applicable

Proquanil

Combination therapy with atovaquone for malaria treatment and prevention

Sulfonamide

Sulfadiazine

Malaria and toxoplasmosis

Not applicable

Pyrimethamine

Combination therapy with sulfadoxine (sulfa drug) for malaria

Produces damaging reactive oxygen species

Not applicable

Artemisinin

Combination therapy to treat malaria

Inhibit DNA synthesis

Nitroimidazoles

Metronidazole, tinidazole

Infections caused by Giardia lamblia, Entamoeba histolytica, and Trichomonas vaginalis

Not applicable

Pentamidine

African sleeping sickness and leishmaniasis

Inhibit heme detoxification

Quinolines

Chloroquine

Malaria and infections with E. histolytica

Mepacrine, mefloquine

Malaria

Table 10.8

  • List two modes of action for antiprotozoan drugs.

Antihelminthic Drugs

Because helminths are multicellular eukaryotes like humans, developing drugs with selective toxicity against them is extremely challenging. Despite this, several effective classes have been developed (Table 10.9). Some bind to helminthic β-tubulin, preventing microtubule formation. Microtubules in the intestinal cells of the worms seem to be particularly affected, leading to a reduction in glucose uptake. Another type of drug binds to glutamate-gated chloride channels specific to invertebrates including helminths, blocking neuronal transmission and causing starvation, paralysis, and death of the worms. Many antihelminthic drugs have modes of action that are unclear, but appear to interfere with ATP formation, inhibit RNA synthesis, or result in calcium influx into the worm.

Common Antihelminthic Drugs

Mechanism of Action

Drug Class

Specific Drugs

Clinical Uses

Inhibit microtubule formation, reducing glucose uptake

Benzimidazoles

Mebendazole, albendazole

Variety of helminth infections

Block neuronal transmission, causing paralysis and starvation

Avermectins

Ivermectin

Roundworm diseases, including river blindness and strongyloidiasis, and treatment of parasitic insects

Inhibit ATP production

Not applicable

Niclosamide

Intestinal tapeworm infections

Induce calcium influx

Not applicable

Praziquantel

Schistosomiasis (blood flukes)

Inhibit RNA synthesis

Thioxanthenones

Lucanthone, hycanthone, oxamniquine

Schistosomiasis (blood flukes)

Table 10.9

 

  • Why are antihelminthic drugs difficult to develop?

Antiviral Drugs

Unlike the complex structure of fungi, protozoa, and helminths, viral structure is simple, consisting of nucleic acid, a protein coat, viral enzymes, and, sometimes, a lipid envelope. Furthermore, viruses are obligate intracellular pathogens that use the host’s cellular machinery to replicate. These characteristics make it difficult to develop drugs with selective toxicity against viruses.

Many antiviral drugs are nucleoside analogs and function by inhibiting nucleic acid biosynthesis. The mode of action of other antivirals is not entirely clear, but it appears that some prevent viral escape from the cell endosome, preventing viral replication, while others block uncoating of the viral particles inside the host cells. Neuraminidase inhibitors (i.e. Tamiflu) specifically target influenza viruses by blocking the activity of influenza virus neuraminidase, preventing the release of the virus from infected cells. These antivirals can decrease flu symptoms and shorten the duration of illness.

Viruses with complex life cycles, such as HIV, can be more difficult to treat. First, HIV targets CD4-positive white blood cells, which are necessary for a normal immune response to infection. Second, HIV is a retrovirus, meaning that it converts its RNA genome into a DNA copy that integrates into the host cell’s genome, thus hiding within host cell DNA. Third, the HIV reverse transcriptase lacks proofreading activity and introduces mutations that allow for rapid development of antiviral drug resistance. To help prevent the emergence of resistance, a combination of specific synthetic antiviral drugs is typically used to treat HIV with antiretroviral therapy (ART), including a reverse transcriptase inhibitora protease inhibitor, and an integrase inhibitor. (Figure 10.8).

Table 10.10 shows the various therapeutic classes of antiviral drugs, categorized by mode of action, with examples of each.

Antiretroviral therapy (ART) is typically used for the treatment of HIV. The targets of drug classes currently in use are shown here.
Figure 10.8 Antiretroviral therapy (ART) is typically used for the treatment of HIV. The targets of drug classes currently in use are shown here. (credit: modification of work by Thomas Splettstoesser)
Common Antiviral Drugs

Mechanism of Action

Drug

Clinical Uses

Nucleoside analog inhibition of nucleic acid synthesis

Acyclovir

Herpes virus infections

Azidothymidine/ zidovudine (AZT)

HIV infections

Ribavirin

Hepatitis C virus and respiratory syncytial virus infections

Vidarabine

Herpes virus infections

Sofosbuvir

Hepatitis C virus infections

Non-nucleoside noncompetitive inhibition

Etravirine

HIV infections

Inhibit escape of virus from endosomes

Amantadine, rimantadine

Infections with influenza virus

Inhibit neuraminadase

Olsetamivir, zanamivir, peramivir

Infections with influenza virus

Table 10.10

Common Antiviral Drugs

Mechanism of Action

Drug

Clinical Uses

Inhibit viral uncoating

Pleconaril

Serious enterovirus infections

Inhibition of protease

Ritonavir

HIV infections

Simeprevir

Hepatitis C virus infections

Inhibition of integrase

Raltegravir

HIV infections

Inhibition of membrane fusion

Enfuviritide

HIV infections

Table 10.10

 

  • Why is HIV difficult to treat with antivirals?

Link to Learning

To learn more about the various classes of antiretroviral drugs used in the ART of HIV infection, explore each of the drugs in the HIV drug classes provided by US Department of Health and Human Services at this (https://openstax.org/l/22HIVUSDepthea) website.

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